For many life science research and clinical testing laboratories, rt PCR test the microplate approach to solution manipulation and analytical measurement is their main choice. Nowadays, the lab has a much-increased need for a higher productivity level, and this is a real concern for laboratory managers.
With the use of microplates, the sample throughput is vastly enhanced. The microplate can allow 96, 384, or even a greater number of samples that can be handled in parallel, allowing for a significant increase in data output. In addition, with the design now being standardized, operations can now be much more automated. Besides the fact that reproducibility is grown through the use of automation, labor cost per analysis is also greatly reduced.
In all major facilities in the drug discovery field, automated high output screening systems use microplates. When the number of wells in each plate is increased, the volume in each well is lowered, thus there is a great desire to increase the number of wells in each plate.
Most wells that are being developed today have about 1536 wells. Most commonly, microplate liquid handling operations have to do with adding or taking away liquid from a well. The way in which this is done is pretty easy to understand, but this isn’t the way it is for stirring the contents of a microplate good, even more so in the newer wells of high capacity plates.
A patent is a monopoly granted by the US government on inventions. Hoffman-La Roche, a Swiss pharmaceutical giant recognized the commercial potential for PCR and purchased the family of PCR patents for $300 million in 1990. The original Mullis patent expired a few years ago, but a “picket fence” of other patents still protects various aspects of the method. Hoffman-La Roche licensed the technology broadly and has developed many of its own products based upon the method. The return on investment of their $300 million has been huge!
This is where the alligator microplate tumble stirrer comes in. Using a stirring method that is much like the way an alligator tumbles its victims, it is used for stirring deep well plates, U and V bottom microplates, PCR plates, and lots of other things used in the lab.
Microplates are made using many different materials:
Usually, polystyrene is used for optical detection microplates. Polypropylene is used to make plates that have wide variances in temperature, like with thermal cycling. It has needed properties for the long-term storage of new chemical compounds.
For the PCR method of DNA amplification, polycarbonate is simple to mold and inexpensive. Most of the time, microplates are made using injection molding, and this is used for polystyrene, polypropylene, and cyclo-olefin. For softer plastics, vacuum forming is often used.
Composite microplates, like filter plates and SPE plates, and even advanced rt pcr test at home plate types, use many parts that are molded separately and then assembled later into the complete product. This is for the scientist, as it makes it possible to only use one part of a plate, which saves money.
Stirrers for microplates are available in a vast array of sizes to match the different well sizes. They can be either made of stainless steel or Parylene coated. Stainless steel stirrers are very resistant to corrosion, and they are not toxic to microorganisms.
They are cheap and can be thrown away after use if the user doesn’t wish to wash them. Parylene coated stirrers are commonly used for combinatorial chemistry since the iron that is found in stainless steel might interfere with the reaction. They are resistant to organic solvents, acids, bases, and are not toxic to tissues.